Whole genome sequencing of SARS-CoV-2 from wastewater links to individual cases in catchments.

After a limited first wave of community transmission in March 2020 and until 2022, Western Australia was largely free of COVID-19, with cases restricted to hotel quarantine, commercial vessels, and small, infrequent community clusters. Despite the low case load setting, sequencing of wastewater samples from large municipal treatment plants produced SARS-CoV-2 genomes with coverage up to 99.7 % and depth to 4000×, which was sufficient to link wastewater sequences to those of active cases in the catchment at the time. This study demonstrates that ≤5 positive individuals can be enough to produce high genomic coverage (>90 %) assemblies even in catchments of up to a quarter of a million people. Genomic analysis of wastewater contemporaneous with clinical cases can also be used to rule out transmission between cases in different catchments, when their SARS-CoV-2 genomes have distinguishing nucleotide polymorphisms. These findings reveal a greater potential of wastewater WGS to inform outbreak management and disease surveillance than previously recognized.

Gratitude for Categories of Needs Before and During the COVID-19 Pandemic.

This naturalistic observation study investigated the influence of broad societal events such as the COVID-19 pandemic on public expressions of gratitude. Spontaneously produced gratitude expressions posted by individuals (N = 159) in an online discussion forum were extracted at three time periods (during the pandemic, one year pre-pandemic, and 2 years pre-pandemic). The gratitude expressions were coded for the categories of deficiency and growth needs based on Maslow's Theory of Motivation. The results demonstrate a higher frequency of gratitude expressions for growth opportunities during the COVID-19 pandemic compared to 2 years prior. The results also demonstrate a higher frequency of gratitude for the fulfillment of deficiency needs compared to growth needs within each of the years, highlighting the overall salience of this category. These findings reveal the capacity of broad societal events to impact public gratitude expressions for needs fulfilment, which has implications for policies and programs intended to meet needs during a global crisis.